Biomaterial system for delivery of immunosuppressive dendritic cells for islet allograft transplant survival and acceptance
A postdoctoral fellow is sought to work on a research project in Dr. Julia Babensee’s laboratory at Georgia Tech that will investigate the use of a hydrogel to deliver islet antigen-specific immunosuppressive dendritic cell (DCs) to support immune acceptance/tolerance towards islet allografts in diabetic mice. The goal is to restore normo-glycemia in diabetic mice. This is a multidisciplinary project involving biomaterials, islet transplantation for diabetes treatment and analysis of immune outcomes. The biomaterial system, available in collaboration with Dr. Andres Garcia (Georgia Tech), is a protease-degradable, in situ crosslinkable hydrogel material that allows for cell encapsulation for their delivery in vivo by injection. The postdoctoral fellow with also work with Dr. Andrew Adams and his associates at the Emory Transplant Center to carry out the islet transplantation studies and assessment of glycemic and immune status of recipients.
The project will develop expertise in experimental design, biomaterial hydrogel formulation, immune cell and islet cell culture and assessment, hydrogel delivery of islets and DCs to mice, analysis of subsequent islet cell function/survival and immune outcomes. Specific techniques to be used include isolation and culture of islets of Langerhans from mice, injection of hydrogel delivering islets and DCs, analysis of islet survival (e.g. histology/immunohistochemistry for insulin, glucose levels), host tissue harvesting for flow cytometric analysis of immune cell phenotype and cytokine profiles. This project is supported by an Innovation & Leadership in Engineering Technologies and Therapies (ILET2) for Diabetes Training funded by NIH. This postdoctoral fellow working on this project will do so at both the Petit Biotechnology Building at Georgia Tech and Emory Transplant Center.
Interested candidates should have as Ph.D. in bio- or biomedical engineering or a basic science (e.g., biochemistry, immunology, biology). Experience in at least some of the following techniques is preferred (but not necessary): mammalian cell culture, ELISA, flow cytometry, working with mice, Luminex, histology/immunohistochemistry. The ability to contribute productively in a multidisciplinary team environment is essential, as is the ability to communicate effectively orally and in writing.
Due to funding restrictions, applicants should be US citizens or permanent residents of the US.
Interested applicants should forward by e-mail a cover letter, curriculum vitae, a short description of career goals and the names, telephone numbers and e-mail addresses of three references to:
Julia E. Babensee, Ph.D.
Wallace H. Coulter Department of Biomedical Engineering
315 Ferst Dr.
Atlanta, GA, 30332-0535