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Rich Carpenedo

Research

Many common methods for directed embryonic stem cell differentiation rely on the application of soluble reagents (e.g. growth factors and small molecules) to embryoid body cultures. In order to improve the homogeneity of differentiation factors presented to cells within embryoid bodies, we are incorporating biodegradable polymer microspheres with encapsulated morphogenic factors within the interior of embryoid body microenvironments. This strategy may improve the homogeneity of differentiation by presenting biomolecular cues in a spatially and temporally controlled manner more uniformly to embryoid body cells.

Selective Awards

2nd Place, Student Poster Award, TERMIS-NA (2008)
1st Place Poster Award, Georgia Life Sciences Summit (2007)
George Fellowship (2007-2009)
GAANN CD4 Graduate Fellowship (2005-2006)
NSF Graduate Fellowship Honorable Mention (2005)

For full list, please see CV

Publications

Carpenedo RL, Seaman SA, McDevitt TC. Microsphere size effects on embryoid body incorporation and embryonic stem cell differentiation. Journal of Biomedical Materials Research Part A, in press (2009).

Sargent CY, Berguig GY, Kinney MA, Hiatt LA, Carpenedo RL, Berson RE, McDevitt TC. Hydrodynamic modulation of embryonic stem cell differentiation by rotary orbital suspension culture. Biotechnology and Bioengineering, in press (2009).

Carpenedo RL, Bratt-Leal AM, Marklein RA, Seaman SA, Bowen NJ, McDonald JF, McDevitt TC. Homogeneous and organized differentiation within embryoid bodies induced by microsphere-mediated delivery of small molecules. Biomaterials, 30(13): 2507-2515 (2009).

Bratt-Leal AM, Carpenedo RL, McDevitt TC. Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. Biotechnology Progress, 25(1): 43-51 (2009).

Carpenedo RL, Sargent CY, McDevitt TC. Rotary suspension culture enhances the efficiency, yield and homogeneity of embryoid body differentiation. Stem Cells 25(9): 2224-2234 (2007).

Book Chapters

Carpenedo RL, McDevitt TC. “Microscale technologies for engineering of embryonic stem cell environments.”  Micro- and Nanoengineering of the Cell Microenvironment: Technologies and Applications (A. Khademhosseini, J Borenstein, S Takayama and M. Toner, eds), pp. 231-243 (2008).

Selective Presentations

Carpenedo RL, Bratt-Leal AM, Seaman SA, Bowen NJ, McDonald JF, McDevitt TC.  Microsphere-mediated Delivery of Retinoic Acid within Embryoid Bodies Induces an Early Streak Phenotype. Poster Presentation, TERMIS-NA, San Diego, CA, December 7-10, 2008. 2nd place poster winner.

Carpenedo RL, Seaman, SA, McDevitt TC. Microsphere Mediated Delivery of Retinoic Acid Directs Embryonic Stem Cell Differentiaiton. Podium Presentation, World Biomaterials Congress, Amsterdam, Netherlands, May 28-June 1, 2008.

Carpenedo RL, Marklein RA, Seaman SA, McDevitt TC.  Microsphere-Mediated Delivery of Retinoic Acid to Embryoid Bodies Alters Embryonic Stem Cell Differentiation.  Poster Presentation, Georgia Life Sciences Summit, Atlanta, GA, October 3, 2007. ***1st place poster winner***

Carpenedo RL, Marklein RA, McDevitt TC. Microsphere Mediated Delivery of Differentiation Factors for Directed Embryonic Stem Cell Differentiation.  Podium Presenation, Society for Biomaterials, Chicago, IL, Oral Presentation, April 18-21, 2007.

Carpenedo RL, Marklein RA, McDevitt TC. Microparticle Mediated Delivery of Differentiation Factors to Embryonic Stem Cells. Poster Presentation, 11th Annual Hilton Head Workshop, Hilton Head, SC, March 7-11, 2007.

Carpenedo RL, McDevitt TC. Controlled Release Biomaterials for Directed Embryonic Stem Cell Differentiation. Poster Presentation, Society for Biomaterials, Pittsburgh, PA, April 26-29, 2006.

For full list, please see CV

Biography

Rich received his bachelor’s degree in biomedical engineering from Case Western Reserve University in 2004.   As an undergraduate, he performed research in the Department of Macromolecular Science and Engineering on the synthesis and characterization of novel benzoxazine-based polymers.  He is currently pursuing a Ph.D. in Bioengineering from the Georgia Institute of Technology in Todd McDevitt’s laboratory.